Journal: Arthritis and rheumatism

Article Title: Mechanisms and clinical relevance of TRAIL-triggered responses in the synovial fibroblasts of patients with rheumatoid arthritis.

doi: 10.1002/art.30181

Figure Lengend Snippet: Figure 4. Rheumatoid arthritis fibroblast-like synoviocytes (RA FLS) produce low levels of the inflammatory cytokines interleukin-6 (IL-6) and IL-8 when stimulated with TRAIL. RA FLS (n 5) were treated with TRAIL (1 nM) or tumor necrosis factor (TNF; 0.5 nM) for 72 hours, and supernatants were analyzed for IL-6 (A), IL-8 (B), and RANTES (C) secretion by enzyme-linked immunosorbent assay. Cy- tokine levels were analyzed using Wilcoxon’s test. Values are the mean SEM. NS not stimulated.

Article Snippet: Serum levels of TRAIL and OPG were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) (Quantikine human OPG ELISA Kit [R&D Systems] and a human TRAIL ELISA [Diaclone]).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Internal and Emergency Medicine

Article Title: Serum TRAIL predicts severity and prognosis in patients with community-acquired pneumonia: a prospective cohort study

doi: 10.1007/s11739-022-03086-7

Figure Lengend Snippet: The levels of circulatory TRAIL in CAP patients with different severity. A-F The levels of circulatory TRAIL were detected through ELISA in CAP patients with different severity scores. A The levels of circulatory TRAIL in CAP patients with different CRB-65 scores. B The levels of circulatory TRAIL in CAP patients with different CURB-65 scores. C The levels of circulatory TRAIL in CAP patients with different SMART-COP scores. D The levels of circulatory TRAIL in CAP patients with different CURXO scores. E The levels of circulatory TRAIL in CAP patients with different PSI scores. F The levels of circulatory TRAIL in CAP patients with different APACHE II scores. * P < 0.5, ** P < 0.01

Article Snippet: TRAIL (CSB-E13164h) and interleukin-1β (IL-1β) (CSB-E08053h) ELISA kits were purchased from Cusabio, Wuhan, China ( https://www.cusabio.com/ ).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: The Journal of Biological Chemistry

Article Title: Toll-like Receptor 3 (TLR3) Induces Apoptosis via Death Receptors and Mitochondria by Up-regulating the Transactivating p63 Isoform α (TAP63α) *

doi: 10.1074/jbc.M110.178798

Figure Lengend Snippet: TNFα and IFNβ do not trigger cell apoptosis. A, poly(I-C) up-regulated the expression of TNFα in immortalized HUVECs. Cells were treated with the indicated concentrations of poly(I-C) for 24 h. The supernatant was harvested to detect the TNFα secretion by ELISA. Data are represented as mean ± S.D. of triplicates. *, p < 0.05 compared with the medium control. B, TNFα (37 °C for 24 h) did not induce cell apoptosis in immortalized HUVECs. C, TNFα (50 ng/ml) induced NF-κB signaling in immortalized HUVECs. D, TNFα neutralization did not inhibit poly(I-C)-induced cell apoptosis in immortalized HUVECs. E, IFNβ (37 °C for 24 h) did not induce cell apoptosis in immortalized HUVECs. F, IFNβ neutralization did not inhibit poly(I-C)-induced cell apoptosis in immortalized HUVECs.

Article Snippet: Human TRAIL ELISA kit was purchased from Boster Biochemicals (Wuhan, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Neutralization

Journal: The Journal of Biological Chemistry

Article Title: Toll-like Receptor 3 (TLR3) Induces Apoptosis via Death Receptors and Mitochondria by Up-regulating the Transactivating p63 Isoform α (TAP63α) *

doi: 10.1074/jbc.M110.178798

Figure Lengend Snippet: TRAIL-DR4/5 and Noxa trigger the extrinsic and intrinsic pathways, respectively. A and B, RT-PCR results show that poly(I-C) (37 °C for 24 h) up-regulated the gene expression of TRAIL, DR4, and DR5 in immortalized (A) and 1 μg/ml poly(I-C) pretreated primary (B) HUVECs. C, poly(I-C) up-regulated the protein expression of TRAIL in primary HUVECs. Cells, pretreated with 1 μg/ml poly(I-C), were re-treated with the indicated concentrations of poly(I-C) for 24 h. TRAIL in the cell lysates was assayed by ELISA. *, p < 0.05 compared with the control. D, TRAIL neutralization repressed the cell apoptosis induced by poly(I-C) in immortalized HUVECs. *, p < 0.05 compared with the poly(I-C) treatment group. E and F, RT-PCR results show the effect of poly(I-C) (37 °C for 24 h) on the gene expression of Bcl-2 and Noxa in immortalized (E) and primary (F) HUVECs. G and H, Western blot results show the effect of poly(I-C) on the protein expression of Bcl-2 and Noxa in immortalized (G) and primary (H) HUVECs. I, inhibition of TLR3 repressed the poly(I-C)-induced down-regulation of Bcl-2 and up-regulation of Noxa in immortalized HUVECs. Cells were transiently transfected with human TLR3 shRNA plasmid and then treated with 2 μg/ml poly(I-C) for 24 h. The protein expression of TLR3, Bcl-2 and Noxa was detected by Western blot.

Article Snippet: Human TRAIL ELISA kit was purchased from Boster Biochemicals (Wuhan, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Gene Expression, Expressing, Enzyme-linked Immunosorbent Assay, Control, Neutralization, Western Blot, Inhibition, Transfection, shRNA, Plasmid Preparation